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Acute stress induced depressive-like behavior and PFC neuronal damage in mice. ( A ) Body weight of the mice in the CON and RS groups over the initial three-day period. Data are expressed as mean ± SEM, with statistical significance assessed via two-factor repeated measures ANOVA. ( B ) Serum <t>corticosterone</t> levels in the two groups of mice. ( C ) Representative movement trajectories of the two groups of mice during the open field test. Red lines show the mice’s movement in the central zone, and brown lines indicate its trajectory in the peripheral zone. ( D , E ) In the open field test, the percentage of movement distance within the central area ( D ) and the percentage of residence time in the central area ( E ). ( F ) The percentage of immobile time in the tail suspension test. n = 6. ( G , H ) Representative images of HE staining ( G ) and Nissl staining ( H ) of the PFC in the two groups of mice. Scale bars: 200 μm and 50 μm. ( I , J ) The relative protein expression levels of TF and TFR in the PFC. n = 6. ( K – M ) The MDA content ( K ), Fe 2+ content ( L ), and GSH content ( M ) in the PFC of the two groups of mice. n = 4. Data were presented as mean ± SEM. Statistical significance between the two groups was assessed using Student’s t -test for normally distributed data, and the Mann–Whitney rank sum test for data that did not follow a normal distribution. Significance levels were denoted as * p < 0.05 and ** p < 0.01.
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Acute stress induced depressive-like behavior and PFC neuronal damage in mice. ( A ) Body weight of the mice in the CON and RS groups over the initial three-day period. Data are expressed as mean ± SEM, with statistical significance assessed via two-factor repeated measures ANOVA. ( B ) Serum <t>corticosterone</t> levels in the two groups of mice. ( C ) Representative movement trajectories of the two groups of mice during the open field test. Red lines show the mice’s movement in the central zone, and brown lines indicate its trajectory in the peripheral zone. ( D , E ) In the open field test, the percentage of movement distance within the central area ( D ) and the percentage of residence time in the central area ( E ). ( F ) The percentage of immobile time in the tail suspension test. n = 6. ( G , H ) Representative images of HE staining ( G ) and Nissl staining ( H ) of the PFC in the two groups of mice. Scale bars: 200 μm and 50 μm. ( I , J ) The relative protein expression levels of TF and TFR in the PFC. n = 6. ( K – M ) The MDA content ( K ), Fe 2+ content ( L ), and GSH content ( M ) in the PFC of the two groups of mice. n = 4. Data were presented as mean ± SEM. Statistical significance between the two groups was assessed using Student’s t -test for normally distributed data, and the Mann–Whitney rank sum test for data that did not follow a normal distribution. Significance levels were denoted as * p < 0.05 and ** p < 0.01.
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Acute stress induced depressive-like behavior and PFC neuronal damage in mice. ( A ) Body weight of the mice in the CON and RS groups over the initial three-day period. Data are expressed as mean ± SEM, with statistical significance assessed via two-factor repeated measures ANOVA. ( B ) Serum <t>corticosterone</t> levels in the two groups of mice. ( C ) Representative movement trajectories of the two groups of mice during the open field test. Red lines show the mice’s movement in the central zone, and brown lines indicate its trajectory in the peripheral zone. ( D , E ) In the open field test, the percentage of movement distance within the central area ( D ) and the percentage of residence time in the central area ( E ). ( F ) The percentage of immobile time in the tail suspension test. n = 6. ( G , H ) Representative images of HE staining ( G ) and Nissl staining ( H ) of the PFC in the two groups of mice. Scale bars: 200 μm and 50 μm. ( I , J ) The relative protein expression levels of TF and TFR in the PFC. n = 6. ( K – M ) The MDA content ( K ), Fe 2+ content ( L ), and GSH content ( M ) in the PFC of the two groups of mice. n = 4. Data were presented as mean ± SEM. Statistical significance between the two groups was assessed using Student’s t -test for normally distributed data, and the Mann–Whitney rank sum test for data that did not follow a normal distribution. Significance levels were denoted as * p < 0.05 and ** p < 0.01.
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Acute stress induced depressive-like behavior and PFC neuronal damage in mice. ( A ) Body weight of the mice in the CON and RS groups over the initial three-day period. Data are expressed as mean ± SEM, with statistical significance assessed via two-factor repeated measures ANOVA. ( B ) Serum <t>corticosterone</t> levels in the two groups of mice. ( C ) Representative movement trajectories of the two groups of mice during the open field test. Red lines show the mice’s movement in the central zone, and brown lines indicate its trajectory in the peripheral zone. ( D , E ) In the open field test, the percentage of movement distance within the central area ( D ) and the percentage of residence time in the central area ( E ). ( F ) The percentage of immobile time in the tail suspension test. n = 6. ( G , H ) Representative images of HE staining ( G ) and Nissl staining ( H ) of the PFC in the two groups of mice. Scale bars: 200 μm and 50 μm. ( I , J ) The relative protein expression levels of TF and TFR in the PFC. n = 6. ( K – M ) The MDA content ( K ), Fe 2+ content ( L ), and GSH content ( M ) in the PFC of the two groups of mice. n = 4. Data were presented as mean ± SEM. Statistical significance between the two groups was assessed using Student’s t -test for normally distributed data, and the Mann–Whitney rank sum test for data that did not follow a normal distribution. Significance levels were denoted as * p < 0.05 and ** p < 0.01.
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Acute stress induced depressive-like behavior and PFC neuronal damage in mice. ( A ) Body weight of the mice in the CON and RS groups over the initial three-day period. Data are expressed as mean ± SEM, with statistical significance assessed via two-factor repeated measures ANOVA. ( B ) Serum <t>corticosterone</t> levels in the two groups of mice. ( C ) Representative movement trajectories of the two groups of mice during the open field test. Red lines show the mice’s movement in the central zone, and brown lines indicate its trajectory in the peripheral zone. ( D , E ) In the open field test, the percentage of movement distance within the central area ( D ) and the percentage of residence time in the central area ( E ). ( F ) The percentage of immobile time in the tail suspension test. n = 6. ( G , H ) Representative images of HE staining ( G ) and Nissl staining ( H ) of the PFC in the two groups of mice. Scale bars: 200 μm and 50 μm. ( I , J ) The relative protein expression levels of TF and TFR in the PFC. n = 6. ( K – M ) The MDA content ( K ), Fe 2+ content ( L ), and GSH content ( M ) in the PFC of the two groups of mice. n = 4. Data were presented as mean ± SEM. Statistical significance between the two groups was assessed using Student’s t -test for normally distributed data, and the Mann–Whitney rank sum test for data that did not follow a normal distribution. Significance levels were denoted as * p < 0.05 and ** p < 0.01.
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Acute stress induced depressive-like behavior and PFC neuronal damage in mice. ( A ) Body weight of the mice in the CON and RS groups over the initial three-day period. Data are expressed as mean ± SEM, with statistical significance assessed via two-factor repeated measures ANOVA. ( B ) Serum corticosterone levels in the two groups of mice. ( C ) Representative movement trajectories of the two groups of mice during the open field test. Red lines show the mice’s movement in the central zone, and brown lines indicate its trajectory in the peripheral zone. ( D , E ) In the open field test, the percentage of movement distance within the central area ( D ) and the percentage of residence time in the central area ( E ). ( F ) The percentage of immobile time in the tail suspension test. n = 6. ( G , H ) Representative images of HE staining ( G ) and Nissl staining ( H ) of the PFC in the two groups of mice. Scale bars: 200 μm and 50 μm. ( I , J ) The relative protein expression levels of TF and TFR in the PFC. n = 6. ( K – M ) The MDA content ( K ), Fe 2+ content ( L ), and GSH content ( M ) in the PFC of the two groups of mice. n = 4. Data were presented as mean ± SEM. Statistical significance between the two groups was assessed using Student’s t -test for normally distributed data, and the Mann–Whitney rank sum test for data that did not follow a normal distribution. Significance levels were denoted as * p < 0.05 and ** p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: The Gut Microbiota Metabolite Butyrate Modulates Acute Stress-Induced Ferroptosis in the Prefrontal Cortex via the Gut–Brain Axis

doi: 10.3390/ijms26041698

Figure Lengend Snippet: Acute stress induced depressive-like behavior and PFC neuronal damage in mice. ( A ) Body weight of the mice in the CON and RS groups over the initial three-day period. Data are expressed as mean ± SEM, with statistical significance assessed via two-factor repeated measures ANOVA. ( B ) Serum corticosterone levels in the two groups of mice. ( C ) Representative movement trajectories of the two groups of mice during the open field test. Red lines show the mice’s movement in the central zone, and brown lines indicate its trajectory in the peripheral zone. ( D , E ) In the open field test, the percentage of movement distance within the central area ( D ) and the percentage of residence time in the central area ( E ). ( F ) The percentage of immobile time in the tail suspension test. n = 6. ( G , H ) Representative images of HE staining ( G ) and Nissl staining ( H ) of the PFC in the two groups of mice. Scale bars: 200 μm and 50 μm. ( I , J ) The relative protein expression levels of TF and TFR in the PFC. n = 6. ( K – M ) The MDA content ( K ), Fe 2+ content ( L ), and GSH content ( M ) in the PFC of the two groups of mice. n = 4. Data were presented as mean ± SEM. Statistical significance between the two groups was assessed using Student’s t -test for normally distributed data, and the Mann–Whitney rank sum test for data that did not follow a normal distribution. Significance levels were denoted as * p < 0.05 and ** p < 0.01.

Article Snippet: Corticosterone (E-OSEL-M0001, Elabscience, Wuhan, China), IL-1β (SEA563Mu, Cloud-clone, Wuhan, China), and IL-6 (SEA079Mu, Cloud-clone, Wuhan, China) levels in mouse serum were quantified using commercial ELISA kits.

Techniques: Suspension, Staining, Expressing, MANN-WHITNEY